The isatin molecule is present in many natural substances, like plants and animals, and it is utilized to prepare compounds with various biological activities. A series of Schiff and Mannich bases derived from isatin were synthesized in this research. First, acetylenic Mannich bases (IIa-e) were prepared by a reaction of isatin with propargyl bromide and different secondary amines (morpholine, piperidine, pyrrolidine, dimethylamine, and diphenylamine) separately, and cuprous chloride (CuCl) was used as a catalyst. Second, these Mannich bases were treated with 2-aminothiazole to obtain the desired final compounds (AM1-AM5). The synthesized compounds were characterized on the basis of their spectral (FT-IR

Hypertension is identified as one of the most significant risk factors for cardiovascular diseases (CVDs). There is growing evidence showing that oxidative stress plays a major role in hypertension. Increased production of reactive oxygen species and decrease bioavailability of antioxidant have been demonstrated in experimental and human hypertension. The present study was directed to determine the beneficial effect of the antioxidant vitamin C in patients with essential hypertension treated with the calcium channel blocker (amlodipine) or with the angiotensin converting enzyme inhibitor (enalapril). Ninety six patients (50 females and 46 males) with essential hyp

Spiro-5-nitro isatino six memberd ring (quinazoline-4-one, thiazine-4-one and oxazine-4-one) respectively were produced by a cycloaddition of 5-nitro isatin Schiff bases [1–5] with anthranilic acid, o-mercapto benzoic acid and salicylic acid. 1HNMR and 13CNMR nuclear magnetic resonance spectroscopies, as well as Fourier-transform infrared spectroscopy, were used to identify the structures of the obtained compounds. These spiro-5-nitro isatin are of interest to us due to their potential antioxidant and anticancer properties. The MTT assay ((3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)) was used to examine in vitro bioactivity testing against breast cancer (MCF-7) cell lines which compound 14 (IC50=79.85,

Abstract:                                                                                                                Fluoroquinolones drugs are an important class of wide

Rheumatoid arthritis is a chronic systemic inflammatory disease. Inflammation leads to joint damage and increases the risk of cardiovascular diseases. Neutrophil lymphocyte ratio (NLR) is a measure of inflammation in many diseases. Therefore, we aimed to evaluate the usefulness of NLR to detect inflammation in RA, and its correlation to RA disease activity indices and some hematological parameters. A cross-sectional study involving 24 patients with active rheumatoid arthritis (RA) who are using MTX participated in this study. All patients were clinically evaluated using disease activity score of 28 joints (DAS28) and simplified disease activity index (SDAI), whereas functional disability was assessed by health assessment questionnaire di

Carnosine have several important biological activities, as implicated by its effects on several diseases such cardiovascular disease, diabetes mellitus, cancer and can play important role in improving the functional capability during ischemic circumstances. Carnosine levels in diabetes patients may be affected by genetic variants in the carnosinase-2 gene, which may also be linked to development of cardiovascular problems. The purpose of this study was to examine the impact of the Carnosinase-2 gene polymorphism SNP (rs 6566810) on serum levels of carnosine and carnosinase-2 and their relationship to CVD in type 2 diabetes (T2DM) patients with and without CVD compared to healthy controls

Diabetes mellitus and insulin resistance cause harm to the liver. In this study, ameliorative effect of Enhydra fluctuans on liver damage in alloxan-induced diabetic rats were examined.

Thirty acclimatized male albino rats divided into six groups (n = 5). Normal rats were fed a standard diet (Group 1); diabetic rats were provided standard feed (Group 2); diabetic rats were fed standard feed and glibenclamide (Group 3); and diabetic rats were fed standard food and fractions of n-hexane E. fluctuans 57.03, 114.06, and 171.09 mg/kg, respectively (Group 4, 5 and 6). For 21 days, rats were fed the n-hexane fraction orally. On day 1 and day 21 of treatments, blood sugar levels were measured. The histophatology of the

Folic acid modulates several disorders in humans, pregnancy mostly because of the mitochondria-rich placenta, is a condition that favors oxidative stress.Transitional metals, especially iron, which are particularly abundant in the placenta, are important in the production of free radicals. Adenosine deaminase (ADA), an enzyme essential for the differentiation of lymphoid cells, has been used for monitoring diseases with altered immunity. In the present study we investigated the effect of regular and irregular administered folic acid in the first and second trimester of pregnancy to be compared with non-pregnant women ,and also the effect of regular and irregular using of folic acid  supplement on  oxidative stress parameters an

Laryngoscopy and tracheal intubation are considered the most invasive stimuli in anesthesia. They provoked cardiovascular responses that include hypertension, tachycardia and dysrhythmias. Various pharmacological approaches have been used to blunt or attenuate such pressor responses. The present study was designed to evaluate the effect of medazolom, lignocaine and propranolol as a valuable adjuvant to fentanyl in attenuating hemodynamic responses to endotracheal intubation in normotensive patients. Thirty two patient with physical status I or II according to the score of American Society of Anesthesiologist (ASA), scheduled for elective surgery under standard general anesthesia, were randomly allocated into four groups (8 patients in ea

Free radicals are reactive compounds, their excessive production is considered to be an important cause of oxidative damage in biomolecules causing degenerative diseases. Polyphenols are one of the most important groups of secondary metabolites of plants, which have an antioxidant activity depending on their properties as hydrogen donors. Echinops polyceras Boiss. (Asteraceae) is one of Echinops genus species that spread in Syria, Lebanon, and Palestine. Phytochemicals found in this species leaves have been extracted with gradient polarity solvents, and primary screening of the secondary metabolites was established. The phenolic compounds and flavonoids contents were determined. The free radicals scavenging activity was evaluated for all

         Recently, emulgel has emerged as one of the most interesting topical preparations in the field of pharmaceutics. In this research clotrimazole was formulated as topically applied emulgel ; different formulas were prepared. The prepared emulgels were evaluated for their physical appearance , rheological behaviour , and in vitro drug release . The influence of the type of gelling agent (carbopol 934 and methyl cellulose), the concentration of both the emulsifying agent (2% and 4% w/w of mixture of span 20 and tween 20) and the oil phase (5% and 7.5% w/w of liquid paraffin) and the type of oil phase (liquid paraffin and cetyl alcohol), on the drug release from the prepared emulgels was invest

Pathogenic microorganisms are becoming more and more resistant to antimicrobial agents. So the synthesis of new antimicrobial agents is very important. In this work, new 5-fluoroisatin-chalcone conjugates 5(a–g) were synthesized based on previous research that showed the modifications of the isatin moiety led to the synthesis of many derivatives that have antimicrobial activity. 4-aminoacetophenone reacts with 5-fluoroisatin to form Schiff base (3), which in turn reacts with two different groups of aromatic (carbocyclic and heterocyclic) aldehydes 4(a–g) separately to form the final compounds 5(a–g). Proton-nuclear

Abstract

      A thin film refers to a pliable polymer layer, with or without a plasticizer, that has been observed to enhance the onset of medicine action, reduce dosing frequency, and enhance the efficacy of drugs. One such technique involves the utilization of polyelectrolyte complexes (PEC) in the preparation of films. This work concerned with studying variables affecting the preparation of topical film for penciclovir using PEC of chitosan and acacia. In this study, Skin bioadhesive polymeric films were fabricated using a solvent casting approach, using acacia gum and chitosan as the main components. The resulting films were further examined to evaluate their features and drug relea

Despite decades of advanced medical study, extracts from coniferous plants have recently been found to be effective against tumor disorders. One of the most prevalent cancers in the world is lung cancer in which every two and a half minutes, lung cancer is discovered in the world. Portulacaria afra Jacq. (spekboom or pork bush) is a medicinal plant belonging to the family of Didieraceae, that contains (7) genera and (20) species .The plant's historical advantages and mythical significance include the usage as a diuretic, for sores, for persistent rash, for heartburn, and for rheumatism. Additionally, it has been claimed that P. afra exhibits antibacterial, antifungal, antioxidant anti-inflammatory and cancer-pr

Rheumatoid arthritis (RA) is a chronic inflammatory disease associated with decreased antioxidant state .This study aim to investigate the status of oxidant/antioxidant in a sample of  Iraqi patients with RA and the role of peroxynitrite and its natural scavenger uric acid in them .This case-controlled study was conducted at Baghdad teaching hospital /Baghdad from December 2010-May 2011 . Twenty-five patients with mean age 39 years and 25 apparently healthy subject as controls with mean age 29 years were included in the study .Investigations include estimation of serum levels of nitric oxide (NO)  ,peroxynitrite (PN) , malondialdehyde (MDA)  , and uric acid (UA) .Serum PN levels were significantly elevated in RA patients a

Electrospinning scaffolds composed of antibiotics and natural polymers can be helpful in the simultaneous suppression of infectious procedures and inducing tissue healing in the treatment of bone infections. The present study was performed to prepare and determine antimicrobial effects, biocompatibility, and osteogenic effects of gelatin meropenem/vancomycin -mesoporous silica nanoparticles (Mrp/Van-MSNPs) prepared using an electrospinning procedure. The scaffolds were investigated for physicochemical features, antimicrobial, biocompatibility, and osteogenic properties. The scaffolds showed applicable mechanical features, slow drug release patterns, and showed antimicrobial effects for 21 days. Gelatin-Mrp/Van-MSNPs showed higher antimic

Asthma is one of the most common chronic, non-communicable diseases affecting children worldwide. The estimated prevalence of pediatric asthma in Iraq is 15.8%. Physiologic, inflammatory and structural factors contribute to the development of asthma. Assessment and monitoring of asthma control can be done by a validated children asthma control test (CACT). Management of asthma must address three components which are an appropriate management plan, the most appropriate medication if necessary, and the use of safe and effective medication. The management plan should consider patient counseling and education about the definition of asthma, signs, and symptoms, the pathophysiology of asthma, common triggers for asthma and how can avoid them,

Salmonella is approved as a common foodborne pathogen, causing major health problems throughout the world particularly in low‐ and middle‐income countries. Low-level fluoroquinolone resistance is conferred by both chromosomal and plasmid-encoded resistance, this research was carried out look into the occurrence rate of qnrA,qnrB and qnrS genes  in  Salmonella enterica serotype Typhi Cipr  ofloxacin-resistant insulate from blood samples of patients with typhoid fever. Fifteen Salmonella enterica serotype Typhi isolated previously from patients with typhoid fever were included in this study. All bacterial isolates were confirmed to have ciprofloxacin

The intestinal mucositis define as inflammation and ulceration in the gastrointestinal tract wall and in some case in the oral cavity these cause by treatment with antineoplastic drug like 5-fluorouracil and Irinotecan and other types of chemotherapeutics drugs , 5-Fluorouracil-induced intestinal mucositis (IM) is consider as one of the more common tumor issue .it cause series of undesirables symptoms like severe diarrhea ,abdominal pain , stomach uncomfortable and other. The aim of this current study to see how ellagic acid act to  Attenuates 5-FU-Induced Intestinal Mucositis and  Diarrhea in Mice . we induced the intestinal mucositis by injected the mice intraperitoneally in 5-fluorouracil about 50mg per kg daily for

   The most frequently diagnosed condition in women at the age of reproduction is the polycystic ovarian syndrome (PCOS).it could be related to a complex endocrine condition, due to its heterogeneity and uncertainty about its etiology, as the clinical highlights of PCOS incorporate those related to reproductive signs such as decreased frequency of ovulation, irregular menstrual cycles, decreased fertility. Carnitine plays a substantial role in weight loss, glucose tolerance, insulin function and fatty acid metabolism. Thus carnitine plays a crucial role in controlling  obesity, insulin resistance, oxidative stress that are  associated with PCOS .While, AGEs are a diverse group of reactive molecules that are formed end

The plant Papaver rhoeas ,which belongs to family Papaveraceae and known as common poppy is wildly grown in Iraq .It was used in traditional medicine in wide range of diseases including inflammation, diarrhea, sleep disorders, treatment of cough, analgesia, and also to reduce the withdrawal signs of opioid addiction.

The project provide the first comprehensive research done in Iraq to study the phytochemical and the methods of extraction and separation of alkaloids from Papaver rhoeas wildly grown in Iraq .The plant was harvested in April 2019 from Zurbatiya is an Iraqi town located at the northeast of Waist province in Iraq.The collected plant was washed thoroughly, dries under shade, and grounding in a mechani

One of the main obstacles to early pregnancy and assisted reproduction is embryo implantation failure. Endometrial receptivity and the interactions between the embryo and the mother determine implantation. The control of implantation involves several molecules. One such molecule is microRNA (miRNA), which is known to have a role in embryo implantation as a transcriptional regulator of gene expression. The current study evaluates the Expression level of miR-210 and miR-1226 with mucin-16 in infertile females under the IVF program and its influence on embryo implantation by measuring the fold change. This study included 26 successful implantations and 58 failed implantations under an in vitro fertilization program and 44 fertile females as

    The plant Abrus precatorius, which belong to Leguminosae (Fabaceae) family and known as Crab’s eyes, Rosary pea with characteristic red and black seeds. It was used in folk medicine in India, China and East Asian countries for treatment of various diseases.

   The plant was extracted by '' general method of extraction'' (Harborne, 1973) using 80% aqueous ethanol as a solvent of extraction by soxhlet apparatus. Preliminary qualitative phytochemical screening were performed on the crude ethanolic extract  and revealed the presence of alkaloids, flavonoids ,terpenoids and phytosterols in Iraqi Abrus pr

Spanlastics are nanovascular drug delivery systems that use surfactants, including hydrophilic and hydrophobic medicines . Spanlastics enhance the ability of medicines to enter the body and provide a continuous release over extended periods . These vesicles possess a high degree of elasticity and flexibility , enabling to improve the transportation of medications through different methods of administrations . Box-behnken design was utilized to establish a relationship between the formulation parameters, specifically the amount of Span 60 , cremophor EL 40 and sonication time and important quality attributes , namely particle size , polydispersity index (PDI) and entrapment efficiency (EE%) . The spanlastic formulations were developed usi

The aim of this study is to formulate and evaluate ezetimibe nanoparticles using solvent antisolvent technology. Ezetimibe is a practically water-insoluble drug which acts as a lipid lowering drug that selectively inhibits the intestinal absorption of cholesterol and related phytosterols. Ezetimibe prepared as nano particles in order to improve its solubility and dissolution rate.

Thirty formulas were prepared and different stabilizing agents were used with different concentrations such as poly vinyl pyrrolidone (PVPK-30), poly vinyl alcohol (PVA), hydroxy propyl methyl cellulose E5 (HPMC), and poloxamer. The ratios of drug to stabilizers used to prepare the nanoparticles were 1: 2, 1:3 and 1:4.

The prepared nanoparticles

This study was designed to compare the effect of two types of viral hepatitis A and E (HAV
and HEV) on liver functions in Iraqi individuals by the measurement of biochemical changes
associated with hepatitis.
The study performed on 58 HEV and 66 HAV infected patients compared with 28 healthy
subjects. The measured biochemical tests include total serum bilirubin, serum transminases (ALT
and AST) alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT).
The study showed that adolescent and young adults (17-29) years, were mostly affected by
HEV while children (5-12) years were frequently affected by HAV. The severity of liver damage in
HEV patients was higher than HAV patients as a result of high serum transa

Interleukins (IL-2 and IL-4) are increased in asthmatics and were reported to induce resistance to steroid therapy in some patients who fail to get benefit from glucocorticoids when used in full dose and for long period of time. In this context, the present study was conducted on Iraqi patients to provide additional laboratory mean, beside the clinical diagnosis, for the decision whether the asthma is steroid sensitive or resistant by monitoring the level of immunoglobulins, complement proteins and interleukins among asthmatic patients (steroid sensitive or resistant) and the possible contribution of other factors like age, sex and environments in the development of steroid resistance. A total number of 55 asthmatics and 28 normal subjec

Abstract

Multiple sclerosis (MS) is a central nervous system disease that causes demyelination and persistent inflammation and most of them among young adults.

The current study aimed to assess gene polymorphism of PDCD1 and PDLCD1 genes and the frequency of two SNPs (rs2227981 and rs2282055).

MS patients (n=100) were divided into two groups; newly diagnosed (42), and patients with ongoing treatments (58). These groups were compared to healthy subjects (n=55); the mean age ±SD was (30±8.46 years), (37±8.06years), and (31±8.73 years) for MS newly patients, patients with ongoing tre

Alkaloids are a group of naturally occurring chemical compounds that contain mostly basic nitrogen atoms . They are a large family of compounds synthesized by plants in addition to the bacteria, fungi, and animals,  they often have pharmacological effects. The aim of this study is to isolate and identified alkaloids in a newly studied, wild Iraqi plant named

The antihypertensive medication olmesartan medoxomil (OLM) is pharmacologically selective  angiotensin-II receptor antagonist. Pharmaceutically speaking, OLM is a class II medication (low solubility, high permeability) that is practically insoluble.Due to its extremely poor solubility, which negatively affects its usefulness, oral medication have a low (26%) bioavailability. The current strategy involves generating OLM as a micellar dispersion in the nano range scale utilizing the film hydration process. In order to prepare transparent aqueous formulations, increase drug solubilization, and administer medication orally, Soluplus (SLP) was employed as a micellar nanocarrier.Different SLP concentrations were used to make eight formula