Workers in automotive repair shops are at risk of exposure to hazardous chemicals, which can have varying health consequences depending on the duration and intensity of exposure, and may ultimately contribute to environmental pollution. The objective of this study is to identify variations in the biochemical levels, including liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid profile, and atherogenic indices (AIP) between workers in car mechanics workshops and occupationally unexposed participants in Baghdad. Blood samples were taken from 70 workers aged 18–50 at car mechanics workshops. The occupations included car maintenance (WI, n = 35), vehicle painting (WII, n = 35), and healthy males (C, n = 25) as the control group. All samples were analyzed for ALT, AST, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and very low-density lipoprotein (VLDL) using commercially available kits. The atherogenic index of sera (AIP) was calculated from values of HDL and TG. The results of the present study revealed a statistically significant increase (p < 0.05) in ALT and AST levels among car maintenance and vehicle painting workers compared with the control group. Significant differences (p < 0.05) were also observed in HDL, LDL, TC, TG, and AIP between the workers and C groups. The study found that liver enzymes, lipid profiles, and indicators of atherogenicity were changed in workshop workers. The workers' exposure to environmental pollution in auto repair workshops was identified as a contributing factor to liver damage, dyslipidemia, and cardiovascular disease in these individuals.