ObjectivePlatanus (plane tree) flowers are rich in condensed tannins (CTs), which have been reported to possess various bioactive properties. However, their specific anti-cancer effects on triple-negative breast cancer cells remain poorly characterized. This study aimed to evaluate the dose-dependent effects of purified Platanus flower CTs on cellular proliferation, migration, and the expression of key malignant-related genes (PCNA and MMP9) in MDA-MB-231 breast cancer cells. Materials and methodsMDA-MB-231 cells were treated with serial concentrations of purified Platanus flower CTs (1.25, 2.5, and 5 mg/mL). Cellular proliferation was assessed by calculating doubling time using time-lapse microscopy and ImageJ single-cell tracking. Migration was evaluated using a wound-healing assay. Gene expression levels of Proliferating Cell Nuclear Antigen (PCNA) and Matrix Metallopeptidase 9 (MMP9) were measured by RT-qPCR. Cell division rates and wound-healing migration were continuously monitored using the Axio Imager time-lapse system. Statistical analyses were conducted using GraphPad Prism softwareResultsTime-lapse analyses showed a clear dose-dependent response. Higher CT concentrations (2.5 and 5 mg/mL) induced complete cell death within the first hour. Treatment at 1.25 mg/mL produced a potent cytostatic effect without acute toxicity, significantly extending the doubling time from approximately 18 hours (control) to approximately 22 hours (P < 0.05). The wound-healing assay revealed significantly reduced wound closure (65-75%) after 24 hours compared to control (80-95%; P < 0.05). Molecular analysis demonstrated that 1.25 mg/mL CTs downregulated PCNA expression by 65% and MMP9 expression by 40%. The pattern of differential gene expression observed herein is fully concordant with both proliferation and migration assays. ConclusionsPurified condensed tannins isolated from Platanus flowers demonstrate significant anti-malignant activity against MDA-MB-231 triple-negative breast cancer cells through a dual mechanism: suppressing proliferation via PCNA downregulation and inhibiting migration via MMP9 downregulation. These findings support the potential of Platanus flower CTs as promising candidates for anti-cancer drug development.
The Influence of Some Vitamins and Biochemical Parameters on Iraqi Females’ Patients with Malignant Breast Cancer"
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In this study, silver nanoparticles (AgNPs) were synthesized using a cold plasma technique and a plasma jet. They were then used to explore how photothermal treatment may be used to treat lung cancer (A549) and normal cells (REF) <i>in vitro</i>. The anti-proliferative activity of these nanoparticles was studied after A549 cells were treated with (AgNPs) at various concentrations (100%, 50%, or 25%) and exposure times (6 or 8 min) of laser after 1 h or 24 h from exposed AgNPs. The highest growth inhibition for cancer cells is (75%) at (AgNPs) concentration (100%) and the period of exposure to the laser is (8 min). Particle size for the prepared samples varied according to the diameter o
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Background: Radiation therapy has the ability to destroy healthy cells in addition to cancer cells in the area being treated. However, when radiation combines with doxorubicin, it becomes more effective on breast cancer treatment. Objective: This study aims to clarify the effect of X-ray from LINAC combined with amygdalin and doxorubicin on breast cancer treatment, and the possibility of using amygdalin with X-ray instead of doxorubicin for the breast cancer treatment. Method: Two cell lines were used in this study, the first one was MCF-7 cell line and second one was WRL- 68 normal cell line. These cells were preserved in liquid nitrogen, prepared, developed and tested in the (place). The effect of three x-ray doses combined with a
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The effect of Low-Level Laser (LLL) provided by green semiconductor laser with an emission wavelength of 532 nm on of human blood of people with brain and prostate cancer has been investigated. The effect of LLL on white blood cell (WBC), NEUT, LYMPH and MONO have been considered. Platelet count (PLT) has also been considered in this work. 2 ml of blood sample were irradiating by a green laser of the dose of 4.8 J/cm2. The results suggest a potential effect of LLL on WBC, PLT, NEUT, LYMPH, and MONO of people with brain and prostate cancer Key words: white blood cell , platelet , low-level laser therapy
Wheat is rich in sources of fiber, oligosaccharides, and resistant starch, simple carbohydrates which may have a protective role against carcinoma. Additionally, Whole wheat/bran as well includes contains phytochemicals such as flavonoids, lignans, folate, phytosterols, phenolic acids, and tocols. The above phytochemicals suitable forms antioxidant and cholesterol-reducing activities. Phytoestrogens are regarded as especially essential in the preventative measures of hormonally dependent malignancies such as breast cancer (BC). In this study lowered BC risk has been associated with whole grain/bran consumption with an odds ratio (OR=0.24 and 95 %CI=0.10-0.56). Wheat/bran appears to have a reliable protective impact against BC. While intake
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