The object of research is studying Raman scattering technique, photoluminescence and some optical properties of silver nanoparticles created by eco-friendly technique which independent on a long time, effort, energy and high temperatures, and with the highest adsorption capacity in order to achieve a high inhibition to paralyze the activity of the bacterial wall, by achieving the highest surface plasmon resonance (SRR). Silver nanoparticles were prepared using Matricaria Flower extract. Characterization of silver nanoparticles and detection of their effectiveness against microbial using two types of bacteria (Escherichia Coli and Staphylococcus aureus ), these nanoparticles were measured using a number of measurements, X-ray diffraction measurement, Energy Dispersion (EDX), (FESEM), U–V Spectroscopy, Fourier Transform Infrared (FTIR), Photoluminescence (PL) properties of silver nanoparticle at room temperature and Raman scattering spectroscopy were investigated. The Scherrer’s equation was used to calculate the crystallite size of Silver nanoparticles, the average crystallite size is 48.64 nm. The PL spectra of silver nanoparticles exhibit two emission bands: one is in the UV region 350 nm and the other is in the visible region 650 nm. This is roughly identical to the absorption spectrum results. The antimicrobial activity was tested against gram negative bacteria (Escherichia coli) and gram positive bacteria (Staphylococcus aureus). Prepared Ag NPs exhibited inhibitory activity in both types of bacteria strains at best selectivity against gram-negative bacteria. An eco-friendly technique is used for synthesizing technique to produce silver nanoparticles with the expected best application properties. These research results suitable to be use the Silver nanoparticles in sensors and many electronic, electrical, medical and biological applications
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